DNA Release and Extraction

microLYSIS^®

microLYSIS is optimised to lyse the cells most commonly used in the lab. This includes bacterial (e.g. E. coli), yeast (e.g. S. cerevisiae) and human cells (e.g. HeLa). It enables you to produce PCR-ready DNA (including plasmid) in less than 15 minutes. Mix the cells with the microLYSIS solution and thermocycle. The DNA can either be used directly or stored at -20°C for the future.

further information >>

Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
microLYSIS	100 preps (2 x 1ml)	2ML-100	£68	€88
microLYSIS	250 preps (5 x 1ml)	2ML-250	£161	€211
microLYSIS	1000 preps (20 x 1ml)	2ML-1000	£612	€802
microLYSIS	2500 preps (50 x 1ml)	2ML-2500	£1,305	€1,710
20 x microLYSIS	500 preps (1 x 0.5ml)	2TwML-500	£297	€389
20 x microLYSIS	1000 preps (2 x 0.5ml)	2TwML-1000	£582	€763
20 x microLYSIS	2500 preps (5 x 0.5ml)	2TwML-2500	£1,305	€1,710

• citations >>

  • Year	Title	URL
    2012	Safety aspects, genetic diversity and technological characterisation of wild-type Streptococcus thermophilus strains isolated from north Italian traditional cheeses	Link
    2011	Disruption of the gene encoding glutamate dehydrogenase affects growth, amino acids catabolism and survival of Lactobacillus plantarum UC1001	Link
    2011	Characterization of Protease IV in Pseudomonas aeruginosa Clinical Isolates	Link
    2011	Technological, phenotypic and genotypic characterisation of wild lactic acid bacteria involved in the production of Bitto PDO Italian cheese	Link
    2010	Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses	Link
    2010	Reduction of fumarate, mesaconate and crotonate by Mfr, a novel oxygen-regulated periplasmic reductase in Campylobacter jejuni	Link
    2009	Characterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei	Link
    2009	The diversity and distribution of toxigenic Microcystis spp. in present day and archived pelagic and sediment samples from Lake Erie	Link
    2009	Fermented goats’ milk produced with selected multiple starters as a potentially functional food	Link
    2009	A kinetic study of in vitro lysis of mycobacterium smegmatis	Link
    2009	A role for tungsten in the biology of Campylobacter jejuni: tungstate stimulates formate dehydrogenase activity and is transported via an ultra-high affinity ABC system distinct from the molybdate transporter	Link
    2009	The Effect of Hydrogen Peroxide on the Hatch Rate and Saprolegnia spp. Infestation of Channel Catfish Eggs	Link
    2009	Early-onset liver mtDNA depletion and late-onset proteinuric nephropathy in Mpv17 knockout mice	Link
    2009	Population structure and safety aspects of Enterococcus strains isolated from artisanal dry fermented sausages produced in Argentina	Link
    2009	Detection and quantification of airborne inoculum of Sclerotinia sclerotiorum using quantitative PCR	Link
    2009	Report of two unlinked cases of infant botulism in the UK in October 2007	Link
    2008	Comparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens- and non-contact lens-related keratitis	Link
    2008	The Campylobacter jejuni Thiol Peroxidases Tpx and Bcp Both Contribute to Aerotolerance and Peroxide-Mediated Stress Resistance but Have Distinct Substrate Specificities	Link
    2008	Antibiotic resistance and microbial composition along the manufacturing process of Mozzarella di Bufala Campana	Link
    2008	Contribution of the stereospecific methionine sulphoxide reductases MsrA and MsrB to oxidative and nitrosative stress resistance in the food-borne pathogen Campylobacter jejuni	Link
    2008	Evaluation of Prebiotic Potential of Refined Psyllium (Plantago ovata) Fiber in Healthy Women	Link
    2008	Presence of vancomycin and ampicillin-resistant Enterococcus faecium of epidemic clonal complex-17 in wastewaters from the south coast of England	Link
    2007	Bacterial Lysis Aureococcus Anophagefferens CCMP 1784 (Pelagophyceae)	Link
    2007	Phenotypic Characterization of Clonal and Nonclonal Pseudomonas aeruginosa Strains Isolated from Lungs of Adults with Cystic Fibrosis	Link
    2007	Laser microdissection separation of pure spermatozoa populations from mixed cell samples for forensic DNA analysis	Link
    2007	Single step cell lysis/PCR detection of Escherichia coli in an independently controllable silicon microreactor	Link
    2007	Identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations	Link
    2007	Microcystin-Production in Lake Erie: Insight into Past and Present Populations	Link
    2006	Molecular Characterization of Subject-Specific Oral Microflora during Initial Colonization of Enamel	Link
    2006	Direct Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef	Link
    2006	Probiotic properties of vaginal lactic acid bacteria to prevent metritis in cattle	Link
    2006	Application of a novel polyphasic approach to study the lactobacilli composition of sourdoughs from the Abruzzo region (central Italy)	Link
    2006	Laser Microdissection Separation of Pure Spermatozoa from Epithelial Cells for Short Tandem Repeat Analysis	Link
    2006	Survival of Yogurt Bacteria in the Human Gut	Link
    2006	Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis	Link
    2006	Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease	Link
    2006	Type III Secretion System–Associated Toxins, Proteases, Serotypes, and Antibiotic Resistance of Pseudomonas aeruginosa Isolates Associated with Keratitis	Link
    2006	Rapid Succession within the Veillonella Population of a Developing Human Oral Biofilm In Situ	Link
    2005	Monitoring the bacterial population dynamics during fermentation of artisanal Argentinean sausages	Link
    2005	An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens	Link
    2005	PCR–DGGE analysis for the identification of microbial populations from Argentinean dry fermented sausages	Link
    2005	Study of Adhesion and Survival of Lactobacilli and Bifidobacteria on Table Olives with the Aim of Formulating a New Probiotic Food	Link
    2005	Rhizodeposits of Trifolium pratense and Lolium perenne: their comparative effects on 2,4-D mineralization in two contrasting soils	Link
    2005	Phenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations	Link
    2005	Molecular subtyping of clinical isolates of Candida albicans and identification of Candida dubliniensisin Malaysia	Link
    2005	In situ activity of a bacteriocin-producing Lactococcus lactis strain. Influence on the interactions between lactic acid bacteria during sourdough fermentation	Link
    2005	A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4	Link
    2005	Rapid Differentiation and In Situ Detection of 16 Sourdough Lactobacillus Species by Multiplex PCR	Link
    2004	SdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity	Link
    2004	Pseudomonas aeruginosa with LasI Quorum-Sensing Deficiency during Corneal Infection	Link
    2004	Comparative evaluation of PCR assays for the robust molecular detection of Mycobacterium avium subsp. Paratuberculosis	Link
    2004	Electrical-Thermal-Fluid Coupled-Field Analysis of Thermal Characterization for Rapid DNA Cell Lysis Microchips	Link
    2004	Evaluation of an impedance method for subtyping of P seudomonas aeruginosa	Link
    2004	Future Probe-Based Strategies	Link
    2004	l-Serine Catabolism via an Oxygen-Labile l-Serine Dehydratase Is Essential for Colonization of the Avian Gut by Campylobacter jejuni	Link
    2003	Effect of the Environment on Genotypic Diversity of Actinomyces naeslundii and Streptococcus oralis in the Oral Biofilm	Link
    2003	Multilocus restriction typing: a tool for Neisseria meningitidis strain discrimination	Link
    2003	Molecular ecology and evolution of slope species	Link
    2002	Oral yeast carriage in patients with advanced cancer	Link
    2002	Analysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase	Link
    2002	Growth of Campylobacter jejuni Supported by Respiration of Fumarate, Nitrate, Nitrite, Trimethylamine-N-Oxide, or Dimethyl Sulfoxide Requires Oxygen	Link
    2002	Strategies for medium-throughput automated genotyping methods	Link
    2001	Rapid Identification of Yeasts in Positive Blood Cultures by a Multiplex PCR Method	Link
    2001	Specificity and sensitivity of microsatellite markers for the identification of larvae	Link
    2001	One-step isolation of plant DNA suitable for PCR amplification	Link

microLYSIS^®-Plus

microLYSIS-Plus will help you get DNA from even the most difficult sources. It can lyse the toughest bacterial cells and spores as well as yeasts, fungi, moulds, some plant and some animal tissues directly. Mix cells with this more complex lysis solution and thermocycle to release DNA. Unlike DNA released with bead-beating systems, DNA released using microLYSIS-Plus is intact.

• further information >>

  • Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
    microLYSIS-Plus	100 preps (2 x 1ml)	2MLP-100	£68	€88
    microLYSIS-Plus	250 preps (5 x 1ml)	2MLP-250	£161	€211
    microLYSIS-Plus	1000 preps (20 x 1ml)	2MLP-1000	£612	€802
    microLYSIS-Plus	2500 preps (1 x 50ml)	2MLP-2500	£1,305	€1,710
    5 x microLYSIS-Plus	250 preps (5 x 1ml)	2FMLP-250	£161	€211
    5 x microLYSIS-Plus	1250 preps (5 x 1ml)	2FMLP-1250	£765	€1,003

• citations >>

  • Year	Title	URL
    2011	Hsp90 gene, an additional target for discrimination between the potato cyst nematodes, Globodera rostochiensis and G. pallida, and the related species, G. tabacum tabacum	Link
    2011	Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes	Link
    2011	Short communication: Epidemiology and genotyping of Candida rugosa strains responsible for persistent intramammary infections in dairy cows	Link
    2011	Identification of New Single Nucleotide Polymorphism-Based Markers for Inter- and Intraspecies Discrimination of Obligate Bacterial Parasites (Pasteuria spp.) of Invertebrates	Link
    2011	Food for thought: Risks of non-native species transfer to the Antarctic region with fresh produce	Link
    2011	Integrated microfluidic array plate (iMAP) for cellular and molecular analysis	Link
    2011	Serotype distribution of Streptococcus pneumoniae causing invasive disease in the Republic of Ireland	Link
    2011	Integrated DNA purification, PCR, sample cleanup, and capillary electrophoresis microchip for forensic human identification	Link
    2010	Occurrence of Pythium aphanidermatum Root and Collar Rot of Papaya (Carica papaya L.) in Cote d'Ivoire	Link
    2010	Intra-specific diversity in noncoding regions of the mitochondrial DNA of Globodera pallida	Link
    2010	A method for release and multiple strand amplification of small quantities of DNA from endospores of the fastidious bacterium Pasteuria penetrans	Link
    2010	Human, mouse or rat? Species authentication of glioma-derived cell cultures	Link
    2010	L'uso della genotipizzazione molecolare in ambito vegetale	Link
    2009	Steinernema australe n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Isla Magdalena, Chile	Link
    2009	Geomyces destructans sp. nov. associated with bat white-nose syndrome	Link
    2009	Rapid and reliable DNA extraction and PCR fingerprinting methods to discriminate multiple biotypes of the nematophagous fungus Pochonia chlamydosporia isolated from plant rhizospheres	Link
    2009	Monolithic Centrifugal Microfluidic Platform for Bacteria Capture and Concentration, Lysis, Nucleic-Acid Amplification, and Real-Time Detection	Link
    2008	Integrated microfluidic tmRNApurification and real-time NASBA device for molecular diagnostics	Link

DNAMITE^®

DNAMITE is our method for extracting large amounts of high purity DNA. We have kits to extract DNA from animal tissue, plant leaves/petals/seeds, nematodes and bacteria. DNAMITE is quicker than spin-column methods and uses fewer steps. It doesn’t use ethanol, phenol, chloroform or isopropanol, so it’s safer for you and the environment.

• further information >>

  • Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
    DNAMITE Plant Kit#1	100 preps	2PLK1-100	£80	€106
    DNAMITE Plant Kit#2	100 preps	2PLK2-100	£94	€122
    DNAMITE Tissue Kit	100 preps	2TK-100	£107	€140
    DNAMITE Bacterial/Plasmid Kit	100 preps	2BPK-100	£107	€140
    DNAMITE for Difficult Cells	100 preps	2DK-100	£107	€140

• citations >>

  • Year	Title	URL
    2011	Effects of fungicide on growth of Leptosphaeria maculans and L. biglobosa in relation to development of phoma stem canker on oilseed rape (Brassica napus)	Link
    2009	Eight polymorphic microsatellite loci markers for the barnacle Balanus amphitrite (syn. Amphibalanus amphitrite) Darwin 1854	Link
    2008	ECA3, a Golgi-Localized P2A-Type ATPase, Plays a Crucial Role in Manganese Nutrition in Arabidopsis1,[C],[W],[OA]	Link
    2008	Development of three real-time PCR assays to detect peanut allergen residue in processed food products	Link
    2005	The plant P1B-type ATPase AtHMA4 transports Zn and Cd and plays a role in detoxification of transition metals supplied at elevated levels	Link

DNAMITE^® DIRECT

DNAMITE DIRECT is a new formulation designed to generate PCR-ready DNA from larger volume samples. Like our other DNA release and extraction products, the emphasis is on speed and ease of use. This kit has been used on meat, fish and feather samples, generating high quality DNA even suitable for qPCR. Contact us for specific protocols.

• further information >>

  • Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
    DNAMITE DIRECT	50 preps	2DD-50	£69	€91
    DNAMITE DIRECT	100 preps	2DD-100	£133	€173