DNA release and extraction:

Novel solutions for attaining DNA from a variety of organisms, samples and cell types. Designed to circumvent traditional and cumbersome DNA extraction methods that will simplify and accelerate your protocol times. A detailed database of applications can be found in our MiZN selector.

Quality testing:

To ensure that the templates and technologies that you use are reliable and accurate, we have designed a number of reagents to assess DNA and cDNA quality, and thermocycler temperature accuracy.

PCR:

Both standard and elite PCR mastermixes that are also available with pre-mixed agarose loading dyes and ROX passive reference dye. If you are working with a tricky GC-rich template we also provide an effective buffer to help balance your assay.

PCR/DNA Cleanup:

These reagents have been designed to purify DNA fragments of all sizes and concentrations with no spin columns and simple steps for greatly reduced protocol times and downstream accuracy.

DNA Sequencing Buffers:

To improve reads and cut costs with optimized dilutions for a number of sequencing platforms.

Molecular Essentials:

If you just require a key chemical component we also provide a number of essentials from dNTPs to MgCl₂.

Title:

Impact of Shortened Crop Rotation of Oilseed Rape on Soil and Rhizosphere Microbial Diversity in Relation to Yield Decline

URL:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059859

Authors:

Sally Hilton, Amanda J. Bennett, Gary Keane, Gary D. Bending, David Chandler, Ron Stobart and Peter Mills

Journal:

PLOS ONE

Date:

1st April 2013

Application:

PCR

Excerpt:

… fungi or the 16S rRNA gene of bacteria. The PCR master mix contained 47 µl Megamix (Microzone Limited, UK) and 10 ng DNA along with 1 µl of a forward primer and 1 µl of a reverse primer. For fungi 25 pmol of PET labelled …

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MegaMix Product Information

Title:

The effect of clozapine on mRNA expression for genes encoding G protein-coupled receptors and the protein components of clathrin-mediated endocytosis

URL:

http://journals.lww.com/psychgenetics/Abstract/publishahead/The_effect_of_clozapine_on_mRNA

Authors:

Sharp, Sally I.; Hu, Ying; Weymer, Jon F.; Rizig, Mie; McQuillin, Andrew; Hunt, Stephen P.; Gurling, Hugh M.D

Journal:

Psychiatric Genetics

Date:

6th March 2013

Application:

PCR cleanup

Excerpt:

… PCR products were visualized by gel electrophoresis in 3.0% (w/v) agarose and then purified by adding an equal volume of microclean (Microzone Ltd, Haywards Heath, UK), centrifuging for 10min at 13000g, and resuspending in 20 μl water. …

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microCLEAN Product Information

Title:

Impacts of biochar on bioavailability of the fungicide azoxystrobin: A comparison of the effect on biodegradation rate and toxicity to the fungal community

URL:

http://www.sciencedirect.com/science/article/pii/S0045653512015329

Authors:

F Sopeña & GD Bending

Journal:

Chemosphere

Date:

7th March 2013

Application:

PCR

Excerpt:

… PCR was conducted in a volume of 50 μL, containing 46 μL of Megamix (Microzone, Haywards Health, UK), both primers at 25 μM, 1 μL of bovine serum albumin (BSA) and 1 μL of DNA template, and amplification was performed using a GeneAmp PCR System 9700 (Applied …

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MegaMix Product Information

Title:

Contrasting arbuscular mycorrhizal communities colonizing different host plants show a similar response to a soil phosphorus concentration gradient

URL:

http://onlinelibrary.wiley.com/doi/10.1111/nph.12169/full

Authors:

Paul Gosling, Andrew Mead, Maude Proctor, John P. Hammond, Gary D. Bending

Journal:

New Phytologist

Date:

20th February 2013

Application:

PCR

Excerpt:

… (2008) labelled with the fluorescent dyes Hex and 6-FAM, respectively. The reaction mixture was as follows: 4 μl DNA (5–25 nmol μl −1 ), 1 μl of each primer (25 pm concentration) and 44 μl of MegaMix (Microzone Ltd, Haywards Heath, UK). …

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MegaMix Product Information

Title:

Genetic Mapping of Genes Involved in Autosomal Recessive Mental Retardation

URL:

http://prr.hec.gov.pk/Thesis/1188S.pdf

Authors:

Muzammil Ahmad Khan

Journal:

Pakistan Research Repository

Date:

15th January 2013

Application:

PCR cleanup

Excerpt:

… purification was done with microclean (Microzone, UK) in which PCR product was mixed with equal volume of microclean solution and centrifuged at 13000 rpm for 5 minutes followed by removal of supernatant with a pipette. Again centrifuged at 13000 rpm for 1 minute to remove residual solution, and distilled water was added to dissolve the precipitated PCR product.

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microCLEAN Product Information

Title:

Thyroid dyshormonogenesis is mainly caused by TPO mutations in consanguineous community

URL:

http://onlinelibrary.wiley.com/doi/10.1111/cen.12127/abstract

Authors:

Hakan Cangul, Zehra Aycan, Alvaro Olivera-Nappa, Halil Saglam, Nadia A Schoenmakers, Kristien Boelaert, Semra Cetinkaya, Omer Tarim, Ece Bober, Feyza Darendeliler, Veysel Bas, Korcan Demir, Banu K Aydin, Michaela Kendall, Trevor Cole, Wolfgang Högler, V Krishna K Chatterjee, Timothy G Barrett, Eamonn R Maher

Journal:

Clinical Endocrinology

Date:

6th December 2012

Application:

PCR cleanup

Excerpt:

… PCR products were size-checked on 1% horizontal agarose gels and cleaned up using MicroCLEAN (Microzone, Haywards Heath, UK) or gel-extracted using QIAquickTM Gel Extraction kit (Qiagen, Crawley, UK). The purified PCR products were sequenced in both forward and …

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microCLEAN Product Information

Title:

The role of local environment and geographical distance in determining community composition of arbuscular mycorrhizal fungi at the landscape scale

URL:

http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej2012127a.html

Authors:

Christina Hazard, Paul Gosling, Christopher J van der Gast, Derek T Mitchell, Fiona M Doohan and Gary D Bending

Journal:

The ISME Journal – Multidisciplinary Journal of Microbial Ecology

Date:

October 25 2012

Application:

PCR amplification

Excerpt:

… and methods). PCR was conducted in a volume of 25 μl and contained 21 μl of Megamix (Microzone, Haywards Heath, UK), 25 pmol of each primer, 1 μl of bovine serum albumin and 2.5 μl of template DNA. Amplification was ….

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MegaMix Product Information

Title:

Genetic signature of accidental transfer of the peregrine earthworm Pontoscolex corethrurus (Clitellata, Glossoscolecidae) in French Guiana

URL:

http://www.sciencedirect.com/science/article/pii/S1164556312000878

Authors:

L. Dupont, T. Decaëns, E. Lapied, V. Chassany, R. Marichal, F. Dubs, M. Maillot, V. Roy

Journal:

European Journal of Soil Biology

Date:

November–December 2012

Application:

PCR cleanup

Excerpt:

A fragment of the cytochrome c oxidase I gene (COI) was amplified according to Folmer et al. [12]. After purification using Microclean (Microzone Ltd.), sequence reactions were performed using the BigDye Terminator Cycle Sequencing kit V. 1.1 (Applied Bio- systems, Foster City, CA, USA) and sequence data were obtained using an ABI310 automatic sequencer (Applied Biosystems). Sequences were manually aligned using the BioEdit program [13]. We estimated the Kimura 2-parameter (K2P) sequence divergence using the software MEGA V 5.05 [14].

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microCLEAN Product Information