Our DNA amplification products have been made to give you the best chance of success with every reaction. We aim to offer a master mix suitable for almost all occasions.
The MegaMixes are simple to use and contain everything needed for PCR amplification except your primers and template. They are designed to produce optimum yield with minimum non-specific background and to enhance reproducibility. All of our MegaMixes are triple-QC’d to ensure maximum consistency between batches. This robust quality has proven especially popular for our customers working in clinical genetics diagnostics.
Polymerase Chain Reaction
MegaMix
A ready-to-use master mix containing recombinant Taq polymerase. A typical 50 µl MegaMix reaction has a final concentration of 2.5 mM MgCl2 and contains 1 unit of Taq along with all the other fixed components needed – high purity dNTPs, reaction buffer and a superb enzyme stabiliser.
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| Product Name | Unit Size | Cat. number | Cat. Price | Euro Price | Data | MSDS |
|---|---|---|---|---|---|---|
| MegaMix | 5 x 1 ml | 2MM-5 | £63 | €80 |  |  |
| MegaMix | 25 x 1 ml | 2MM-25 | £288 | €378 | | |
| MegaMix Bulk | Box of 100 x 1 ml | 2MM-100 | £954 | €1,250 | | |
| MegaMix Bulk | 2.5 x Box of 100 x 1 ml | 2MM-250 | £2,087 | €2,735 | | |
| MegaMix Bulk | 3 x Box of 100 x 1 ml | 2MM-300 | £2,435 | €3,191 | | |
| MegaMix-Double | 5 x 0.5 ml | 2MMD-5 | £63 | €80 | | |
| MegaMix-Double | 25 x 0.5 ml | 2MMD-25 | £288 | €378 | | |
| MegaMix-Double Bulk | Box of 100 x 0.5 ml | 2MMD-100 | £954 | €1,250 | | |
| MegaMix-Double Bulk | 2.5 x Box of 100 x 0.5 ml | 2MMD-250 | £2,087 | €2,735 | | |
| MegaMix-Double Bulk | 3 x Box of 100 x 0.5 ml | 2MMD-300 | £2,435 | €3,191 | | |
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Year Title URL 2012 Analysis of LIN28A in early human ovary development and as a candidate gene for primary ovarian insufficiency Link 2011 Chromosomal evolution in Gekkonidae. I. Chromosome painting between Gekko and Hemidactylus species reveals phylogenetic relationships within the group Link 2010 Astrocyte-Derived Vascular Endothelial Growth Factor Stabilizes Vessels in the Developing Retinal Vasculature Link 2010 A novel envelope mediated post entry restriction of murine leukaemia virus in human cells is Ref1/TRIM5α independent Link 2010 Modular plastic chip for one-shot human papillomavirus diagnostic analysis Link 2010 ChIP-on-chip analysis reveals angiopoietin 2 (Ang2, ANGPT2) as a novel target of steroidogenic factor-1 (SF-1, NR5A1) in the human adrenal gland Link 2010 Hylotrupes bajulus (L.) (Col., Cerambycidae): nutrition and attacked material Link 2009 Disposable plastic microreactors for genomic analyses Link 2009 Identification of Mutations in the Kir6.2 Subunit of the KATP Channel Link 2009 Erythromycin- and tetracycline-resistant lactobacilli in Italian fermented dry sausages Link 2008 Comparison of Polymerase Chain Reaction, Immunochromatographic Assay and Staining Techniques in Diagnosis of Cryptosporidiosis Link 2008 Impact of antibiotics on the gut microbiota of critically ill patients Link 2008 Azole Resistance and ERG11 464 Polymorphism in Oral Candida albicans Clinical Strains Isolated in Sardinia Link 2007 Intrinsic genetic characteristics determine tumor-modifying capacity of fibroblasts: matrix metalloproteinase-3 5A/5A genotype enhances breast cancer cell invasion Link 2006 Single cell PCR amplification of microsatellites flanking the COL7A1 gene and suitability for preimplantation genetic diagnosis of Hallopeau–Siemens recessive dystrophic epidermolysis bullosa Link 2006 Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva Link 2005 A Pyrosequencing assay for rapid recognition of SNPs in Mycobacterium tuberculosis embB306 region Link 2004 Human platelet antigen genotyping by PCR-SSP in neonatal/fetal alloimmune thrombocytopenia Link 2003 Analysis of GNAS1 and overlapping transcripts identifies the parental origin of mutations in patients with sporadic Albright hereditary osteodystrophy and reveals a … Link 2001 Rapid protein-based assays for the diagnosis of T–B+ severe combined immunodeficiency Link
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Product Name Unit Size Cat. number Cat. Price Euro Price Data MSDS MegaMix-Blue 5 x 1 ml 2MMB-5 £63 €80 MegaMix-Blue 25 x 1 ml 2MMB-25 £288 €378 MegaMix-Blue Bulk Box of 100 x 1 ml 2MMB-100 £954 €1,250 MegaMix-Blue Bulk 2.5 x Box of 100 x 1 ml 2MMB-250 £2,087 €2,735 MegaMix-Blue Bulk 3 x Box of 100 x 1 ml 2MMB-300 £2,435 €3,191 Blue MegaMix-Double 5 x 0.5 ml 2MMBD-5 £63 €80 Blue MegaMix-Double 25 x 0.5 ml 2MMBD-25 £288 €378 Blue MegaMix-Double Bulk Box of 100 x 0.5 ml 2MMBD-100 £954 €1,250 Blue MegaMix-Double Bulk 2.5 x Box of 100 x 0.5 ml 2MMBD-250 £2,087 €2,735 Blue MegaMix-Double Bulk 3 x Box of 100 x 0.5 ml 2MMBD-300 £2,435 €3,191
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Year Title URL 2011 Defective gonadotropin-releasing hormone neuron migration in mice lacking SEMA3A signalling through NRP1 and NRP2: implications for the aetiology of hypogonadotropic hypogonadism Link 2011 A novel form of pigment-dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach) Link 2011 The Porcine Limbal Epithelial Stem Cell Niche as a New Model for the Study of Transplanted Tissue-Engineered Human Limbal Epithelial Cells Link 2011 A SEPALLATA gene is involved in the development and ripening of strawberry (Fragaria×ananassa Duch.) fruit, a non-climacteric tissue* Link 2010 Identification and expression of mRNAs encoding bursicon in the plesiomorphic central nervous system of Homarus gammarus Link 2010 A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse Link 2010 A highly permeable species boundary between two anadromous fishes Link 2010 Defective GnRH neuron migration in mice lacking SEMA3A signalling through NRP1 and NRP2: implications for the aetiology of hypogonadotropic hypogonadism Link 2010 Postglacial Recolonization of Continental Europe by the Pygmy Shrew (Sorex minutus) Inferred From Mitochondrial and Y Chromosomal DNA Sequences Link 2010 Simulation of an in vitro niche environment that preserves conjunctival progenitor cells Link 2010 Changes in fibroblast mechanostat set point and mechano-sensitivity suggest an adaptive response to mechanical stress in Floppy Eyelid Syndrome Link 2010 PRNP allelic series from 19 years of prion protein gene sequencing at the MRC Prion Unit Link 2010 IL6 and the human limbal stem cell niche: A mediator of epithelial–stromal interaction Link 2010 Characterisation and functional features of a spontaneously immortalised human corneal epithelial cell line with progenitor-like characteristics Link 2010 Mismatch primer-based PCR reveals that helicase–primase inhibitor resistance mutations pre-exist in herpes simplex virus type 1 clinical isolates and are not induced during incubation with the inhibitor Link 2009 Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe Link 2009 Conjunctival epithelial cells maintain stem cell properties after long-term culture and cryopreservation Link 2009 Negative regulation of fibroblast growth factor 10 (FGF-10) by polyoma enhancer activator 3 (PEA3) Link 2009 Shadoo (Sprn) and prion disease incubation time in mice Link 2009 Isolation of identical nitrilase genes from multiple bacterial strains and real-time PCR detection of the genes from soils provides evidence of horizontal gene transfer Link 2009 HECTD2 Is Associated with Susceptibility to Mouse and Human Prion Disease Link 2009 Multiple colonisations of the western Indian Ocean by Pteropus fruit bats (Megachiroptera: Pteropodidae): The furthest islands were colonised first Link 2009 Genetic structure of, and hybridisation between, red (Cervus elaphus) and sika (Cervus nippon) deer in Ireland Link 2009 Multiple paternity in the European hedgehog Link 2009 The heritability and genetics of frontotemporal lobar degeneration Link 2008 Evaluation of a New Assay in Comparison with Reverse Hybridization and Sequencing Methods for Hepatitis C Virus Genotyping Targeting Both 5' Noncoding and Nonstructural 5b Genomic Regions Link 2008 Long-term persistence of a single Legionella pneumophila strain possessing the mip gene in a municipal shower despite repeated cycles of chlorination Link 2008 A distinct clinical, neuropsychological and radiological phenotype is associated with progranulin gene mutations in a large UK series Link 2008 Investigation of Mcp1 as a Quantitative Trait Gene for Prion Disease Incubation Time in Mouse Link 2007 Single amino acid substitutions in the HSV-1 helicase protein that confer resistance to the helicase-primase inhibitor BAY 57-1293 are associated with increased or decreased virus growth characteristics in tissue culture Link 2007 Invasive group A streptococcal infection in injecting drug users and non-drug users in a single UK city Link 2007 Selective requirements for NRP1 ligands during neurovascular patterning Link 2007 Transcript profiling of the hypomethylated hog1 mutant of Arabidopsis Link 2007 Differential Requirements for MCM Proteins in DNA Replication in Drosophila S2 Cells Link 2006 Identification and developmental expression of mRNAs encoding crustacean cardioactive peptide (CCAP) in decapod crustaceans Link 2006 Rodrigues fruit bats (Pteropus rodricensis, Megachiroptera: Pteropodidae) retain genetic diversity despite population declines and founder events Link 2006 A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening Link 2005 Phylogeography of the pool frog (Rana lessonae Camerano) in Europe: evidence for native status in Great Britain and for an unusual postglacial colonization route Link 2005 The Drosophila Cdc6/18 protein has functions in both early and late S phase in S2 cells Link 2004 Identification and characterization of a novel mouse prion gene allele Link 2004 Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces Link 2003 Increasing local levels of neuregulin (glial growth factor-2) by direct infusion into areas of demyelination does not alter remyelination in the rat CNS Link 2002 A multiplex allele specific polymerase chain reaction (MAS-PCR) on the dihydrofolate reductase gene for the detection of Cryptosporidium parvum genotypes 1 and 2 Link
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Year Title URL 2008 Spectrum of GJB2 mutations causing deafness in the British Bangladeshi population Link 2007 Spectrum of MKS1 and MKS3 mutations in Meckel syndrome: a genotype-phenotype correlation Link 2005 CDKN2A mutations in Scottish families with cutaneous melanoma: results from 32 newly identified families Link
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Product Name Unit Size Cat. number Cat. Price Euro Price Data MSDS MegaMix-Gold 5 x 1 ml 2MMG-5 £220 €289 MegaMix-Gold 10 x 1 ml 2MMG-10 £426 €559 MegaMix-Gold Bulk Box of 50 x 1 ml 2MMG-50 £1,967 €2,578 MegaMix-Gold Bulk Box of 100 x 1 ml 2MMG-100 £3,737 €4,896 MegaMix-Gold Bulk 2.5 x Box of 100 x 1 ml 2MMG-250 £8,787 €11,518 MegaMix-Gold Bulk 3 x Box of 100 x 1 ml 2MMG-300 £9,887 €12,957
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Year Title URL 2011 Behavioral and Other Phenotypes in a Cytoplasmic Dynein Light Intermediate Chain 1 Mutant Mouse Link 2010 Molecular Genetics External Quality Assessment Pilot Scheme for KRAS Analysis in Metastatic Colorectal Cancer Link 2010 Human, mouse or rat? Species authentication of glioma-derived cell cultures Link
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Product Name Unit Size Cat. number Cat. Price Euro Price Data MSDS Rox H MegaMix-Gold 5 x 1 ml 2RMMGH-5 £220 €289 Rox H MegaMix-Gold 10 x 1 ml 2RMMGH-10 £426 €559 Rox H MegaMix-Gold Bulk Box of 50 x 1 ml 2RMMGH-50 £1,967 €2,578 Rox H MegaMix-Gold Bulk Box of 100 x 1 ml 2RMMGH-100 £3,737 €4,896 Rox H MegaMix-Gold Bulk 2.5 x Box of 100 x 1 ml 2RMMGH-250 £8,787 €11,518 Rox H MegaMix-Gold Bulk 3 x Box of 100 x 1 ml 2RMMGH-300 £9,887 €12,957 Rox L MegaMix-Gold 5 x 1 ml 2RMMGL-5 £220 €289 Rox L MegaMix-Gold 10 x 1 ml 2RMMGL-10 £426 €559 Rox L MegaMix-Gold Bulk Box of 50 x 1 ml 2RMMGL-50 £1,967 €2,578 Rox L MegaMix-Gold Bulk Box of 100 x 1 ml 2RMMGL-100 £3,737 €4,896 Rox L MegaMix-Gold Bulk 2.5 x Box of 100 x 1 ml 2RMMGL-250 £8,787 €11,518 Rox L MegaMix-Gold Bulk 3 x Box of 100 x 1 ml 2RMMGL-300 £9,887 €12,957
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citations >>
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Year Title URL 2010 A Copine family member, Cpne8, is a candidate quantitative trait gene for prion disease incubation time in mouse Link 2009 HECTD2 Is Associated with Susceptibility to Mouse and Human Prion Disease Link 2009 Shadoo (Sprn) and prion disease incubation time in mice Link 2008 Investigation of Mcp1 as a Quantitative Trait Gene for Prion Disease Incubation Time in Mouse Link
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Product Name Unit Size Cat. number Cat. Price Euro Price Data MSDS MegaMix-Royal 5 x 1 ml 2MMR-5 £220 €289 MegaMix-Royal 10 x 1 ml 2MMR-10 £426 €559 MegaMix-Royal Bulk Box of 50 x 1 ml 2MMR-50 £1,967 €2,578 MegaMix-Royal Bulk Box of 100 x 1 ml 2MMR-100 £3,737 €4,896 MegaMix-Royal Bulk 2.5 x Box of 100 x 1 ml 2MMR-250 £8,787 €11,518 MegaMix-Royal Bulk 3 x Box of 100 x 1 ml 2MMR-300 £9,887 €12,957
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Year Title URL 2011 Occurrence of Tropical Race 4 of Fusarium oxysporum f. sp. cubense in Indonesia Link 2010 Alterations in LMTK2, MSMB and HNF1B gene expression are associated with the development of prostate cancer Link 2010 Occurrence of Pythium aphanidermatum Root and Collar Rot of Papaya (Carica papaya L.) in Côte d'Ivoire Link 2009 Steinernema australe n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Isla Magdalena, Chile Link 2009 Phylogenetic Diversity of the Causative Agents of Vibriosis Associated with Groupers Fish from Karimunjawa Islands, Indonesia Link 2008 Phylogenetic diversity of organophosphorous pesticide-degrading coral bacteria from Mid-West coast of Indonesia Link 2008 Succession of Actinomycetes During Composting Proccess of Dairy-Farm Waste Investigated by Culture-Dependent and Independent Approaches Link 2008 Characterization of Psychrotrophic Bacteria from Sea Waters of Makasar Strait, Indonesia Link 2006 Phylogenetic Diversity of Secondary Metabolite Producing-Bacteria Associated with Sponges from Bandengan Waters, Jepara Link
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Product Name Unit Size Cat. number Cat. Price Euro Price Data MSDS microFORCE 1 x 1 ml (200 reactions) 2MFO-1 £19 €25 microFORCE 5 x 1 ml (1000 reactions) 2MFO-5 £79 €105 microFORCE 10 x 1 ml (2000 reactions) 2MFO-10 £133 €174
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MegaMix-Blue
This has all the advantages of MegaMix but also incorporates a blue dye, allowing easy visualisation and eliminating the need for additional gel loading buffers. The dye does not inhibit restriction enzymes or ligases and does not fluoresce at the wavelengths used by automated DNA sequencers. Therefore it doesn’t interfere with the usefulness of the PCR – just makes it more convenient to use.
MegaMix-W
This is a special mix formulated for use with the Wave® Machine (Transgenomics Inc.). Many chemicals found in PCR buffers (e.g. BSA) can interfere with the performance of the Wave® Machine. MegaMix-W does not contain these chemicals and so gives reliable results without the need for further optimisation.
further information >>
| Product Name | Unit Size | Cat. number | Cat. Price | Euro Price | Data | MSDS |
|---|---|---|---|---|---|---|
| MegaMix-W | 5 x 1 ml | 2MMW-5 | £63 | €80 | | |
| MegaMix-W | 25 x 1 ml | 2MMW-25 | £288 | €378 | | |
| MegaMix-W Bulk | Box of 100 x 1 ml | 2MMW-100 | £954 | €1,250 | | |
| MegaMix-W Bulk | 2.5 x Box of 100 x 1 ml | 2MMW-250 | £2,087 | €2,735 | | |
| MegaMix-W Bulk | 3 x Box of 100 x 1 ml | 2MMW-300 | £2,435 | €3,191 | | |
MegaMix-Gold
The first of our elite mixes, MegaMix-Gold is designed to help you succeed with even the most challenging PCR systems. It is supplied double concentrated and contains a mixture of Taq polymerase and monoclonal anti-Taq polymerase antibodies. An initial step of 5 minutes at 95°C denatures the antibodies, thus activating the Taq. Since the Taq is completely inactive at room temperature, samples do not need to be set-up on ice.
Rox MegaMix-Gold
MegaMix-Gold is also available with either high or low concentration of ROX dye. These mixes are for machines which require ROX as a reference.
MegaMix-Royal
Our second elite mix, MegaMix-Royal is a recalibration of MegaMix-Gold formulated to give you a second option. For convenience the same blue dye used with MegaMix-Blue is incorporated so you can load agarose gels straight away. It is supplied double concentrated and uses the same Hot-Start technology as MegaMix-Gold which offers highly consistent results even with challenging PCR systems.
microFORCE® Buffer
For use with GC-rich DNA templates, this convenient 4x buffer has been designed to enhance amplification even further when using our MegaMix-Gold or MegaMix-Royal mixes. Maximise your success with templates of up to 75% GC without inhibiting amplification from those with low GC content.
