DNA Release

microLYSIS^®-PLUS

• An extremely rapid one tube, one reagent method for lysing cells and releasing DNA
  from a variety of different cell types.

• Contains an optimised mixture of detergents and enzymes which integrate into the
  walls of the cells during thermal cycling and cause cell lysis.

• microLYSIS-PLUS very effectively degrades tough cell walls and can therefore release
  the DNA from more difficult tissues such as some plant and animal tissues.

• Suitable for standard and Real-Time PCR.

• Available with MegaMix-ROYAL as part of LYSE 'N EASY for an easier way to get from cells to sequence!

• microLYSIS-PLUS is also available as a 5x concentrate.

Prices >>

Data Sheet >>

microLYSIS^®

• microLYSIS contains the same optimised mixture of detergents as microLYSIS-PLUS but not the enzymes and is therefore ideal for lysing less challenging cells such as bacteria, yeast and mammalian cultured cells.

• microLYSIS is also available as a 20x concentration.

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Data Sheet >>

microLYSIS^® for Infected Blood

• A two step lysis system containing microLYSIS and two pre-lysis solutions.

• The prelysis solutions are 10/1 TE (weak prelysis) and RB (strong prelysis), chosen according to sample preferences and pathogen type.

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Data Sheet >>

DNA Extraction

DNAMITE^®

• An innovative kit for the extraction of DNA from Plant, Tissue, Bacteria and Difficult Cells for subsequent PCR or restriction digest analysis.

• DNAMITE gives the same high DNA purity and yield as spin column competitors, yet takes half the time with fewer steps, reducing human error and the need for costly repeats. Click here for a comparison table.

• DNAMITE is safer for you and the environment. No nasty ethanol, phenol chloroform or isopropanol.

 

Prices >>

Plant Kit #1 Data Sheet >>   Plant Kit #2 Data Sheet >>  

Tissue Kit Data Sheet >>      Bacteria Kit Data Sheet >>  

Difficult Cell Data Sheet >>

 

 

DNA Amplification

MegaMix

• Microzone produces a range of great value, easy to use mastermixes for all kinds of PCRs.

• The MegaMixes are quick and simple to use and contain everything needed for PCR amplification, except your primers and DNA template of course.

• MegaMix has been carefully optimised to produce maximum yield with minimum non specific background.

• MegaMix increases reproducibility of DNA amplification, saves valuable laboratory time and dramatically reduces the risks of contamination during reagent handling.

• MegaMix is very stable and can be frozen and thawed many times without any loss of activity.

• MegaMix is also available as a double concentration, particularly useful when using dilute DNA templates or primers

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Data Sheet >>

 

MegaMix-BLUE

• All the benefits of standard MegaMix but with the extra convenience of a blue agarose loading gel,
  allowing for easy visualisation and accurate small volume pipetting.

• The dye incorporated into the MegaMix -BLUE does not interfere with the usefulness or the further analysis of PCR products. It does not inhibit restriction enzymes or ligases, furthermore the blue dye does not flouresce at the wavelengths used by automated DNA sequencers.

• MegaMix -BLUE is also available as a double concentration.

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Data Sheet >>

 

MegaMix-W

• A special MegaMix formulation for the Wave^® Machine. Any chemicals found in PCR buffers (eg BSA) can interfere with the performance of the Wave^® Machine.

• MegaMix -W does not contain any such chemicals and gives consistant and good results without further optimisation.

• MegaMix -W is also available as a double concentration.

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Data Sheet >>

 

MegaMix-GOLD

• Used for 'difficult' PCRs, MegaMix -GOLD is a double concentrated, Hot-Start Taq mix containing optimised Taq,
  anti-Taq polymerase monoclonal antibodies, 400µM dNTPs and 6 mM MgCl₂ in a buffer with a stabiliser.

• The mix only becomes active when the temperature has reached >90°C.

• The chance of primers forming primer-dimers or unspecific bonds to incorrect DNA sequences is
  thus minimised.

• MegaMix -GOLD is also available with a high or low concentration of ROX reference dye.

Prices >>

Data Sheet >>

 

MegaMix-ROYAL

• The king of the MegaMix castle, highly consistent results even with 'challenging' PCRs.

• MegaMix -ROYAL has all the benefits of MegaMix -GOLD but with a blue agarose gel loading dye for increased convenience.

• MegaMix -ROYAL is also available with microLYSIS-PLUS as part of LYSE 'N EASY for an easier way to get from cells to sequence!

Prices >>

Data Sheet >>

LYSE 'N EASY^®

• An easier way form cells to sequence, a two stage protocol now available in one kit for value and convenience.

• Incorporating microLYSIS-PLUS and MegaMix -ROYAL for easier DNA release and amplification.

• Tried and tested and found to give highly consistent results even when trying to lyse the toughest of cells, including arabidopsis, flour, fungus, fleas etc.

• Click here for the results of our recent 'in house' tests.

Prices >>

Data Sheet >>

 

DNA Clean-Up

microCLEAN

• microCLEAN is specially formulated to capture small DNA.

• Ideal for cleaning up PCR fragments prior to sequencing. Just add equal volume of microCLEAN to the sample and spin.

• microCLEAN offers excellent recovery and gives reliable results, with fewer steps and less stress than spin column competitors. Click here for a comparison table.

• Protocols available for tubes and plates.

Prices >>

Data Sheet >>

CA Solution

• CA Solution is specially formulated to capture large DNA .

• It offers an easy way to clean up and/or concentrate your genomic DNA.

• Clean DNA ready for further processing in a matter of minutes.

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Data Sheet >>

Quality Testing

Human DNAOK!

• HDNAOK! is a multiplex PCR kit containing everything required for testing Human DNA extractions enabling you to estimate the quality and quantity of Human DNA before undertaking further downstream tests.

• HDNAOK! can also be used as a tool to test for PCR inhibitors in DNA extracts or to compare the effectiveness of different DNA extraction methods.

• Five primer pairs amplify from five different regions of the
  chromosome = G on the gel photo.

• If the DNA is partially degraded or fragmented one or more PCR fragments are missing = B on the gel photo.

• If the DNA is fully degraded all of the five fragments are missing and only the
  positive PCR control is visible = U on the gel photo.

Prices >>

Data Sheet >>

Human cDNAOK!^®

• Microzone developed the cDNAOK! kit to screen cDNA preparations before taking them on further, often very expensive, downstream processes, enabling the removal of any unsuccessful and degraded preparations saving valuable time and money.

• cDNAOK! is a multiplex PCR kit, specially designed to amplify constitutively expressed genes from different chromosomes.
  Careful optimisation when making this kit means that only cDNA is amplified and not any contaminating genomic DNA, therefore giving a true reflection of the quality of the cDNA preparation.

• If the cDNA preparation is good, then all four PCR
  products will be amplified = G on the gel photo.

• If the preparation is weak or the cDNA is degraded
  then the intensity of the bands will decrease = B on the gel photo.

• If there is no cDNA present at all, or it has completely degraded, then
  only the positive control will amplify = U on the gel photo.

 

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Data Sheet >>

Cycler Testing

OK!KIT

• Sometimes when PCRs that have previously worked suddenly just fail it could be that your Thermal Cycler isn't performing properly.
  The OK!KIT Contains all the reagents needed for a quick and easy testing of the performance of your Thermal Cycler.

• Using the OK!KIT to regularly test your Thermal Cycler's performance could highlight quickly why your PCRs aren't working, potentially saving you huge amounts of time and money by preventing wasteful repeats.

• The OK!KIT can identify as little as 1°C temperature change and is therefore a fast, convenient, inexpensive and informative way to monitor the performance of your Thermal Cycler.

G = Good machine: - Top band just visible. Machine working correctly.

B = Bad machine: - Middle and top band too strong.
Machine cooling below the required annealing temp.

U = Ugly machine: - No top band and middle band very faint.
Machine not cooling to the required annealing temp.

 

 

 

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Data Sheet >>

 

DNA Sequencing

BetterBASE and BetterBuffer

• BetterBASE is a novel enhancing buffer specially designed and tested for use with the ABI BigDye^® sequencing mix.

• BetterBASE is compatible for use with all sequencing machines.

• This buffer replaces either home made or other commercial dilution buffers and can provide greatly improved results with difficult templates.

• Simply dilute the ABI BigDye^® v 1.1 and 3.1 mix with BetterBase so you economise and improve your sequencing at the same time!

Other dilution buffers are also available:

BetterBuffer for the ABI machines
BetterBuffer-MB for the MegaBACE™ machines
And BaseQ^® 5x sequencing buffer

 

Prices >>

Data Sheet >>

DNA Size Standards

For Agarose Gels

• Our DNA Size standards cover a wide range of molecular sizes from 50 base pairs through to 23 kilobases.

• All the agarose size standards supplied 'ready to load' in room temperature stable loading buffer.

• Optimal agarose gel concentrations are given for each DNA ladder.

Prices >>

100bp Ladder Data Sheet >>  1kb Ladder Data Sheet >>

Lambda-PstI Data Sheet >>  Lambda-HindIII Data Sheet >>

Lambda-EcorI Data Sheet >>  Lambda-EcorI/HindIII Data Sheet >>

Primer Extension And Building Blocks

 Primer Extension

Tough-Taq

• Tough-Taq polymerase is expressed from a 5' deletion of the gene encoding the Thermus Aquaticus DNA polymerase, resulting in a protein very similar to the Stoffle Fragment.

• It is a highly active and extremely heat stable DNA polymerase, repeated exposure to 98°c in the appropriate reaction buffer does not seem to diminish the enzyme activity.In contrast the full length enzyme will not tolerate such treatment.

• Each kit contains: Tough-Taq 500 or 5000 units, Reaction Buffer Plus (10x), Reaction Buffer Minus (10x), MgCl₂ (1.0M) and Enzyme dilution buffer.

• Tough-Taq is available in two different activities:
  Kit 1 - 25 units/µl
  Kit 2 - 5 units/µl

• This product is sold as Klen Taq1™ in the USA

Prices >>

Reaction Kit 1Data Sheet >>

Reaction Kit 2 Data Sheet >>

Building Blocks

dNTP (set)

• 'Ready to use' molecular grade dNTP solutions in a set of four individual nucleotides for use in DNA polymerisation reactions, DNA labelling and sequencing processes.

• Each set comprises separate vials of ultrapure dATP, dCTP, dGTP and dTTP solutions.

• Available at either 10mM or 100 mM concentrations

10mM Data Sheet >>

100mM Data Sheet >>

dNTP (mix)

• A premixed 'ready to use' molecular grade dNTP solution comprising 2mM dATP, dCTP, dGTP and dTTP for use in PCR applications, DNA labelling and DNA sequencing.

• In a typical 50µl PCR, 5µl of dNTP mix will give a final concentration of 200µM of each dNTP.

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Data Sheet >>

Molecular Grade Reagents

Ethidium Bromide

• ‘Ready to use’ Molecular Biology grade Ethidium Bromide suitable for the efficient visualisation of DNA and RNA following electrophoresis in agarose gels.

• Supplied as a 10 mg/ml solution.

Just Water

• ‘Ready to use’ Molecular Biology grade highly pure water suitable for use in all molecular biology applications.

• Quality control shows the complete absence of DNase and RNase.

TE

• 'Ready to use' Molecular Biology grade 10 mM Tris/1mM EDTA

• Quality control shows the complete absence of DNase and RNase.

MgCl₂ Solution

• ‘Ready to use’ Molecular Biology grade 1M MgCl₂ solution for use in all molecular biology applications.

• Quality control shows the complete absence of DNase and RNase.
  

Mineral Oil

• ‘Ready to use’ Molecular Biology grade highly pure oil suitable for use in all molecular biology applications.

• Quality control shows the complete absence of DNase and RNase.

Prices >>

Data Sheets >>