The easy way to check your PCR machine. Just take 10 μl from two tubes, mix together and thermocycle. The OK!Kit will identify whether your thermocycler is running correctly. It will identify as little as 1°C deviation from the correct annealing temperature and so is a fast, convenient and inexpensive way of assessing machine performance.
In the test, a multiplex PCR reaction is precisely optimised to generate three amplicons of different intensity in thermocyclers that are running correctly as shown in the picture.
Sample 1 (Lane 2) has been produced in a machine running correctly. However, sample 2 (Lane 3) and sample 3 (Lane 4) have been produced in failing machines. Where bands appear of the same intensity (Lane 3), the machine is cooling below the required annealing temperature. Where bands are faint or have disappeared (Lane 4), the machine is not cooling sufficiently to meet the required annealing temperature.
Lane 1 – 100 bp DNA ladder, Lane 2 – Sample 1 generated in thermocycler running correctly, Lane 3 – Sample 2 generated in thermocycler running incorrectly (cooling below desired annealing temp), Lane 4 – Sample 3 generated in thermocycler running incorrectly (not cooling to meet desired annealing temp).
|PRODUCT NAME||UNIT SIZE||CAT. NUMBER||CAT. PRICE||EURO PRICE||DATA||MSDS|
|OK! Kit||100 tests||2OK-100||£57||€74|
|OK! Kit||500 tests||2OK-500||£246||€320|