Quality Testing

Human DNAOK!

This kit enables you to check the quality of your human DNA extractions. Mix extraction samples with the kit’s MegaMix-Gold and primer solutions, thermocycle and visualise on agarose gel. Guard against extraction failures, the presence of PCR inhibitors and DNA degradation following storage.

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Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
Human DNAOK!	150 tests	2HDOK-150	£122	€162
Human DNAOK!	300 tests	2HDOK-300	£239	€313

A multiplex PCR reaction is used to amplify from five different regions of the genome as well as a positive PCR control. If the DNA is good quality, the reaction will produce amplicons from five regions plus the control (see Lane 2 in the Figure opposite). If the DNA is partially degraded or fragmented, one or more of the amplicons from the genome will be missing (Lane 3). If the DNA has been entirely degraded, only the positive control will amplify (Lane 4).

Lane 1 – 100 bp DNA ladder, Lane 2 – High quality intact human DNA, Lane 3 – Partially degraded human DNA, Lane 4 – Fully degraded human DNA (positive PCR control visible).

Human cDNAOK!^®

Like the Human DNAOK! kit, this has been designed to enable you to confirm the quality of the materials you’re working with, so you can have absolute confidence in your results. The primer mix solution amplifies constitutively expressed genes from different chromosomes and is optimised so that only cDNA is amplified and not any contaminating genomic DNA. In this way it provides a true reflection of the quality of the cDNA preparation. Unsuccessful and degraded preps can be quickly identified, saving you valuable time as well as money.

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  • Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
    Human cDNAOK!	150 tests	2HCDOK-150	£122	€162
    Human cDNAOK!	300 tests	2HCDOK-300	£239	€313



The genes are amplified in a multiplex PCR reaction. If the cDNA preparation has been successful and is not degraded, four amplicons from genes will be present as well as a positive control (Lane 2). If the prep was unsuccessful or the cDNA is degraded, bands will be missing (Lane 3). If the prep has failed absolutely, or stored cDNA has completely degraded, only the positive PCR control will be clearly visible (Lane 4).

Lane 1 – 100 bp DNA ladder, Lane 2 – High quality intact human cDNA, Lane 3 – Partially degraded human cDNA, Lane 4 – Fully degraded human cDNA (positive PCR control visible).

OK! Kit

The easy way to check your PCR machine. Just take 10 μl from two tubes, mix together and thermocycle. The OK!Kit will identify whether your thermocycler is running correctly. It will identify as little as 1°C deviation from the correct annealing temperature and so is a fast, convenient and inexpensive way of assessing machine performance.

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  • Product Name	Unit Size	Cat. number	Cat. Price	Euro Price	Data	MSDS
    OK! Kit	100 tests	2OK-100	£56	€72
    OK! Kit	500 tests	2OK-500	£239	€313



In the test, a multiplex PCR reaction is precisely optimised to generate three amplicons of different intensity in thermocyclers that are running correctly as shown in the picture.

Sample 1 (Lane 2) has been produced in a machine running correctly. However, sample 2 (Lane 3) and sample 3 (Lane 4) have been produced in failing machines. Where bands appear of the same intensity (Lane 3), the machine is cooling below the required annealing temperature. Where bands are faint or have disappeared (Lane 4), the machine is not cooling sufficiently to meet the required annealing temperature.

Lane 1 – 100 bp DNA ladder, Lane 2 – Sample 1 generated in thermocycler running correctly, Lane 3 – Sample 2 generated in thermocycler running incorrectly (cooling below desired annealing temp), Lane 4 – Sample 3 generated in thermocycler running incorrectly (not cooling to meet desired annealing temp).