A modified version of microLYSIS® designed to lyse tough tissues such as plant and some animal tissues.
The protocol is the same except for a longer incubation step at 65°c. For VERY tough tissue this step may need to be even longer and can even be used as an overnight incubation if required.
microLYSIS®-PLUS is also available with MegaMix-ROYAL as part of LYSE 'N EASY®, for an easier way to get from cells to sequence!
microLYSIS®-PLUS is also available as a 5x concentrate
A novel reagent specially formulated as a pre-step to PCR in circumstances where purification of the DNA is unnecessary.
Tried and tested 'one step' protocols exist for many cell types including:
Yeast
Bacteria
Viruses
Whole Blood
Buccal
microLYSIS® is also available as a 20x concentration.
Click me for Protocol
A two step lysis system containing microLYSIS® and two pre-lysis solutions. The prelysis solutions are 10/1 TE (weak prelysis) and RB (strong prelysis), chosen according to sample preferences and pathogen type.