Where do you want to get DNA from?
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Human/Animal
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Tissues
For smaller samples, microLYSIS®-Plus can be used to extract PCR-ready DNA from a huge range of animal tissues. From humans, it has been used effectively with a wide range of cell types – for example, sperm, buffy coat, fibroblasts, keratinocytes and HeLa carcinoma cells etc. It has also been used successfully on other mammalian tissue samples, including mouse tails and ears and African Fruitbat wing samples. It produces PCR/qPCR-ready DNA that can be used immediately or stored long-term at -20°C
For larger tissue samples we recommend DNAMITE® DIRECT which is formulated for diagnostic use with meat and fish. This product also rapidly produces PCR/qPCR-ready DNA that can be used straight away or stored at -20°C
If purified DNA from tissues is required we recommend our DNAMITE® Tissue kit or DNAMITE® for Difficult Cells for saliva samples/buccal swabs
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Feathers
We have successfully trialled the use of DNAMITE® DIRECT for extracting PCR-ready DNA from moulted feather samples. The DNA can be stored long-term at -20°C or used immediately
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Small Animals
Our products have been used to obtain DNA from a wide range of small organisms. microLYSIS®-Plus, for example, has been used to generate PCR-ready DNA from adult nematodes, encapsulated nematode larvae (Anguillicoloides crassus), Schistosomes, Drosophila, Tardigrades, Fleas and various species of meiofauna. microLYSIS®-Plus DNA can be used immediately or stored long term at -20°C
Our DNAMITE® for Difficult Cells kit has been used to purify DNA from a similarly wide range of sources too. Perhaps most impressive is purified DNA from whole barnacles
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Plant
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Seeds and Tubers
Our product microLYSIS®-Plus has successfully been used to extract PCR-ready DNA from a variety of seeds and tubers. For example, from peanuts, rice, grass seed – even the starchy endosperm of maize seeds. It can generate PCR-ready DNA in minutes which can be used immediately or stored long-term at -20°C
For purified DNA from seeds/tubers we recommend our DNAMITE® Plant kits. We offer Kit#1 without RNAse and Kit#2 with, depending on your requirements
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Leaves, Stems and Flowers
microLYSIS®-Plus has been used to generate PCR-ready DNA from the leaves, flower petals, pollen and tissues of many crop plants such as lettuce, pepper and paw-paw. It has also been used with research plants such as wheat (Triticum aestivum) and maize (Zea mays) and Arabidopsis thaliana. microLYSIS®-Plus generates PCR-ready DNA which can be used immediately or stored long-term at -20°C
For purified DNA from plant leaves, flowers, pollen and tissue we recommend our DNAMITE® Plant kits. We offer kits with/without RNAse, depending on your needs
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Fungi
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Unicellular fungi
Our customers report that microLYSIS® is effective for DNA release from the unicellular fungi Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida spp. This product releases PCR-ready DNA suitable for immediate use or long-term storage at -20°C. For purified DNA from these species we recommend our DNAMITE® Bacterial/Plasmid kit. If you are trialling DNA release or extraction from a different unicellular fungus, contact our technical support for advice, although in most cases we would expect the kits above to be sufficient
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Fungal spores
microLYSIS®-Plus has been used to produce PCR-ready DNA from the spores of a variety of fungi. It has been used successfully with isolated spores of Phakospora euvitis (Grapevine Leaf Rust), leaf-borne spores of Sclerotinia sclerotiorum (Sclerotinia stem rot in oilseed rape) and with airborne spores, via spore-tape experiments, of the wheat pathogens Microdochium nivale cv. Nivale and Fusarium spp.
microLYSIS®-Plus produces PCR-ready DNA which can be used immediately or stored long-term at -20°C
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Other fungi
For production of PCR-ready DNA we recommend microLYSIS®-Plus. This product has been used with mycelial samples of a wide variety of fungal species. Notable examples include the novel bat pathogen Geomyces destructans and the nematophagus fungus Pochonia chlamydosporia. To produce purified DNA from fungal samples we recommend our DNAMITE® Plant Kit #2
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Protist
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Algae, slime moulds and water moulds
Although we have customers that have used microLYSIS®-Plus to release PCR-ready DNA from the water mould oomycete Pythium aphanidermatum, as yet we have no customers using any of our products to extract DNA from either algae or slime moulds. However, we have tested our microLYSIS®-Plus solution on the slime mould Physarum polycephalum with some success. microLYSIS®-Plus disrupts cells to release DNA that is immediately suitable for PCR and real-time PCR. This PCR-ready DNA can be stored long-term at -20°C
We anticipate that for most unicellular algae and other slime mould species, microLYSIS®-Plus would be sufficient for generating PCR-ready DNA. For more complex algal species, including seaweeds, a modified protocol may be successful or our DNAMITE® for Difficult Cells kit. We would also recommend this kit for those wishing to generate purified DNA from algae or slime moulds
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Cryptosporidium
We have successfully trialled microLYSIS®-Plus for the extraction of PCR-ready DNA from the protozoan Cryptosporidium parvum. This product disintegrates the cell to release PCR-ready DNA which can either be used straight away or stored long term at-20°C. Customers wishing to generate purified DNA should use our DNAMITE® for Difficult Cells kit
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Other Protists
The Protista kingdom is very diverse and our products have, of course, only been used with a tiny fraction of its species. For those organisms where there is not a tried and tested method we would recommend trialling microLYSIS®-Plus for PCR-ready DNA and our DNAMITE® for Difficult Cells kit for producing purified DNA. Alternatively, contact us for technical support and we’ll happily provide advice
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Micro-organism
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Gram-negative bacteria
Our customers report 100% success lysing Gram-negative colony isolates with microLYSIS®. Notable species with which microLYSIS® has been used include Haemophilus influenzae and Neisseria meningitides during epidemiological screens.
microLYSIS® disintegrates cells so that DNA is released (genomic and plasmid) which is immediately suitable for PCR and real-time PCR. This PCR-ready DNA can be stored long-term at -20°C.
If customers require purified DNA from Gram-negative cells we suggest using our DNAMITE® Bacterial/Plasmid kit.
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Gram-positive bacteria
Customers report almost 100% success lysing Gram-positive colony isolates with microLYSIS®-Plus. Notable species include Streptococcus pneumoniae and Bacillus cereus. microLYSIS®-Plus has also been used successfully in a general screen on colonies isolated from seawater, lysing enterococci as well as Bacteroides and coliforms.
One significant advantage of microLYSIS®-Plus over some methods is that it leaves the genomic DNA largely intact. Thus, it has been shown to improve DNA collection from Gram-positive spore-forming micro-organisms where a bead-beating system would be the usual alternative method and when whole genome amplification is the objective.
We always recommend microLYSIS®-Plus first to customers working with Gram-positive bacteria. It can disrupt tough cells to release DNA which is immediately suitable for PCR and real-time PCR. The DNA can also be stored long-term at -20°C. For the few Gram-positives which stubbornly resist this product (for example, DNA yields from S. agalactiae can be a little low) we recommend our DNAMITE® for Difficult Cells kit. We also recommend this kit if customers require purified DNA from gram-positive cells.
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Archaea
As far as we know, we have no customers who specifically work with Archaeal species, so whilst it’s likely that Archaeal species that stain gram-negative could be lysed with microLYSIS®, we recommend microLYSIS®-Plus for obtaining PCR-ready DNA because of the great diversity of Archaeal cell wall structures.
Like microLYSIS®, microLYSIS®-Plus disintegrates cells to release DNA, but unlike the former it is optimised to disrupt even the toughest cells. microLYSIS®-Plus disintegrates cells so that DNA is released (genomic and plasmid) which is immediately suitable for PCR and real-time PCR. This PCR-ready DNA can be stored long-term at -20°C.
For customers that require purified DNA from Archaea we would recommend our DNAMITE® for Difficult Cells, although for Archaea that stain gram-negative we would expect our DNAMITE® Bacterial/Plasmid kit to be sufficient.
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Viruses
Our novel column-free DNA release method microLYSIS®-Plus has been successfully optimised for Baculovirus. It delivers high yield complementing qPCR assays for quantitation of virus titrations to ensure optimal recombinant protein production. This method will reduce the amount of cell handling required and prevent against any delay in the protein production process.
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Alternatively, you can search our citation table for the particular sample or cell type that you are interested in:
Sample / Cell Type Product Protocol Article URL Actinomyces naeslundii microLYSIS according to manufacturer's protocol Effect of the Environment on Genotypic Diversity of Actinomyces naeslundii and Streptococcus oralis in the Oral Biofilm Link
Actinomyces naeslundii microLYSIS according to manufacturer's protocol Phenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations Link
Brevibacterium linens microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Campylobacter jejuni microLYSIS according to manufacturer's protocol Analysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase Link
Campylobacter jejuni microLYSIS according to manufacturer's protocol Contribution of the stereospecific methionine sulphoxide reductases MsrA and MsrB to oxidative and nitrosative stress resistance in the food-borne pathogen Campylobacter jejuni Link
Campylobacter jejuni microLYSIS according to manufacturer's protocol Growth of Campylobacter jejuni Supported by Respiration of Fumarate, Nitrate, Nitrite, Trimethylamine-N-Oxide, or Dimethyl Sulfoxide Requires Oxygen Link
Campylobacter jejuni microLYSIS according to manufacturer's protocol L-Serine Catabolism via an Oxygen-Labile L-Serine Dehydratase Is Essential for Colonization of the Avian Gut by Campylobacter jejuni Link
Campylobacter jejuni microLYSIS according to manufacturer's protocol Reduction of fumarate, mesaconate and crotonate by Mfr, a novel oxygen-regulated periplasmic reductase in Campylobacter jejuni Link
Campylobacter jejuni microLYSIS according to manufacturer's protocol The Campylobacter jejuni Thiol Peroxidases Tpx and Bcp Both Contribute to Aerotolerance and Peroxide-Mediated Stress Resistance but Have Distinct Substrate Specificities Link
Candidatus phytoplasma mali microLYSIS according to manufacturer's protocol Endophytic bacterial community living in roots of healthy and ‘Candidatus Phytoplasma mali’-infected apple (Malus domestica, Borkh.) trees Link
Clostridium botulinum microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec Report of two unlinked cases of infant botulism in the UK in October 2007 Link
Corynebacterium casei microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Enterococcus faecalis microLYSIS according to manufacturer's protocol Characterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei Link
Enterococcus faecalis microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Enterococcus faecium microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Escherichia coli microLYSIS-Plus according to manufacturer's protocol Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics Link
Escherichia coli microLYSIS-Plus 65 °C for 15 min, 96 °C for 2 min, 65 °C for 4 min, 96 ° C for 1 min, 65 °C for 1 min and 96 °C for 30 sec Monolithic Centrifugal Microfluidic Platform For Bacteria Capture and Concentration, Lysis, Nucleic-Acid Amplification, and Real-Time Detection Link
Escherichia coli microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec SdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity Link
Formalin-Fixed Paraffin-Embedded Tissue (FFPE) microLYSIS according to manufacturer's protocol Comparative evaluation of PCR assays for the robust molecular detection of Mycobacterium avium subsp. Paratuberculosis Link
Fungal isolates microLYSIS-Plus according to manufacturer's protocol Food for thought: Risks of non-native species transfer to the Antarctic region with fresh produce Link
Fungal isolates microLYSIS-Plus according to manufacturer's protocol Geomyces destructans sp. nov. associated with bat white-nose syndrome Link
Lactobacilli microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec Fermented goats’ milk produced with selected multiple starters as a potentially functional food Link
Lactobacilli microLYSIS according to manufacturer's protocol In situ activity of a bacteriocin-producing Lactococcus lactis strain. Influence on the interactions between lactic acid bacteria during sourdough fermentation Link
Lactobacilli microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec Rapid Differentiation and In Situ Detection of 16 Sourdough Lactobacillus Species by Multiplex PCR Link
Lactobacilli microLYSIS according to manufacturer's protocol Survival of Yogurt Bacteria in the Human Gut Link
Lactobacilli microLYSIS according to manufacturer's protocol Antibiotic resistance and microbial composition along the manufacturing process of Mozzarella di Bufala Campana Link
Lactobacillus paracasei microLYSIS according to manufacturer's protocol Characterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei Link
Lactobacillus acidipiscis microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Lactobacillus curvatus microLYSIS according to manufacturer's protocol Direct Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef Link
Lactobacillus curvatus microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Lactobacillus delbrueckii microLYSIS according to manufacturer's protocol Survival of Yogurt Bacteria in the Human Gut Link
Lactobacillus plantarum microLYSIS according to manufacturer's protocol Disruption of the gene encoding glutamate dehydrogenase affects growth, amino acids catabolism and survival of Lactobacillus plantarum UC1001 Link
Lactobacillus sakei microLYSIS according to manufacturer's protocol Direct Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef Link
Leuconostoc gelidum microLYSIS according to manufacturer's protocol Direct Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef Link
Leuconostoc mesenteroides microLYSIS according to manufacturer's protocol Characterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei Link
Macrococcus caseolyticus microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Micrococcaceae strains microLYSIS according to manufacturer's protocol Monitoring the bacterial population dynamics during fermentation of artisanal Argentinean sausages Link
Micrococcus luteus microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Microcystis spp microLYSIS according to manufacturer's protocol The diversity and distribution of toxigenic Microcystis spp. in present day and archived pelagic and sediment samples from Lake Erie Link
Mouse tissue microLYSIS according to manufacturer's protocol Early-onset liver mtDNA depletion and late-onset proteinuric nephropathy in Mpv17 knockout mice Link
Mycobacterium bovis microLYSIS (20x) according to manufacturer's protocol An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens Link
Mycobacterium smegmatis microLYSIS according to manufacturer's protocol A Kinetic Study of In Vitro Lysis of Mycobacterium smegmatis Link
Neisseria meningitidis microLYSIS according to manufacturer's protocol Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease Link
Neisseria meningitidis microLYSIS according to manufacturer's protocol Multilocus restriction typing: a tool for Neisseria meningitidis strain discrimination Link
Nematode microLYSIS-Plus (5x) 66 °C for 30 min, 96 °C for 3 min, 65 °C for 4 min, 96 °C for 2 min, 65 °C for 1 min and 96 °C for 1 min; or incubation at 65 °C for 60 min and 94 °C for 10 min Hsp90 gene, an additional target for discrimination between the potato cyst nematodes, Globodera rostochiensis and G. pallida, and the related species, G. tabacum tabacum Link
Plankton microLYSIS according to manufacturer's protocol Microcystin-Production in Lake Erie: Insight into Past and Present Populations Link
Propionibacterium freudenrechii microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Pseudomonas aeruginosa microLYSIS according to manufacturer's protocol Comparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens- and non-contact lens-related keratitis Link
Pseudomonas aeruginosa microLYSIS according to manufacturer's protocol Evaluation of an impedance method for subtyping of Pseudomonas aeruginosa Link
Pseudomonas aeruginosa microLYSIS according to manufacturer's protocol Phenotypic Characterization of Clonal and Nonclonal Pseudomonas aeruginosa Strains Isolated from Lungs of Adults with Cystic Fibrosis Link
Pseudomonas aeruginosa microLYSIS according to manufacturer's protocol Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis Link
Pseudomonas aeruginosa microLYSIS according to manufacturer's protocol Pseudomonas aeruginosa with LasI Quorum-Sensing Deficiency during Corneal Infection Link
Pythium aphanidermatum microLYSIS-Plus 65 °C for 30 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec Occurrence of Pythium aphanidermatum Root and Collar Rot of Papaya (Carica papaya L.) in Cote d'Ivoire Link
Soil microbes microLYSIS according to manufacturer's protocol Rhizodeposits of Trifolium pratense and Lolium perenne: their comparative effects on 2,4-D mineralization in two contrasting soils Link
Spermentoza microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec Laser Microdissection Separation of Pure Spermatozoa Populations from Mixed Cell Samples for Forensic DNA Analysis Link
Staphylococcus aureus microLYSIS according to manufacturer's protocol Development of a real-time quadruplex PCR assay for simultaneous detection of nuc, Panton–Valentine leucocidin (PVL), mecA and homologue mecALGA251 Link
Staphylococcus aureus microLYSIS according to manufacturer's protocol PCR–DGGE analysis for the identification of microbial populations from Argentinean dry fermented sausages Link
Staphylococcus capitis microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec SdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity Link
Staphylococcus caprae microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Staphylococcus epidermidis microLYSIS 65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 sec SdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity Link
Staphylococcus equorum microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Staphylococcus lentus microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Staphylococcus saprophyticus microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Staphylococcus sciuri microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Staphylococcus vitulinus microLYSIS according to manufacturer's protocol Surface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses Link
Streptococcus oralis microLYSIS according to manufacturer's protocol Effect of the Environment on Genotypic Diversity of Actinomyces naeslundii and Streptococcus oralis in the Oral Biofilm Link
Streptococcus pneumoniae microLYSIS-Plus according to manufacturer's protocol Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes Link
Streptococcus thermophilus microLYSIS according to manufacturer's protocol Survival of Yogurt Bacteria in the Human Gut Link
Streptococcus thermophilus microLYSIS according to manufacturer's protocol Safety aspects, genetic diversity and technological characterisation of wild-type Streptococcus thermophilus strains isolated from north Italian traditional cheeses Link
Veillonella microLYSIS according to manufacturer's protocol Rapid Succession within the Veillonella Population of a Developing Human Oral Biofilm In Situ Link