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Where do you want to get DNA from?

  • Selector Human/Animal
    • Tissues

      For smaller samples, microLYSIS®-Plus can be used to extract PCR-ready DNA from a huge range of animal tissues. From humans, it has been used effectively with a wide range of cell types – for example, sperm, buffy coat, fibroblasts, keratinocytes and HeLa carcinoma cells etc. It has also been used successfully on other mammalian tissue samples, including mouse tails and ears and African Fruitbat wing samples. It produces PCR/qPCR-ready DNA that can be used immediately or stored long-term at -20°C

      For larger tissue samples we recommend DNAMITE® DIRECT which is formulated for diagnostic use with meat and fish. This product also rapidly produces PCR/qPCR-ready DNA that can be used straight away or stored at -20°C

      If purified DNA from tissues is required we recommend our DNAMITE® Tissue kit or DNAMITE® for Difficult Cells for saliva samples/buccal swabs

      Contact us for more information
    • Feathers

      We have successfully trialled the use of DNAMITE® DIRECT for extracting PCR-ready DNA from moulted feather samples. The DNA can be stored long-term at -20°C or used immediately

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    • Small Animals

      Our products have been used to obtain DNA from a wide range of small organisms. microLYSIS®-Plus, for example, has been used to generate PCR-ready DNA from adult nematodes, encapsulated nematode larvae (Anguillicoloides crassus), Schistosomes, Drosophila, Tardigrades, Fleas and various species of meiofauna. microLYSIS®-Plus DNA can be used immediately or stored long term at -20°C

      Our DNAMITE® for Difficult Cells kit has been used to purify DNA from a similarly wide range of sources too. Perhaps most impressive is purified DNA from whole barnacles

      Contact us for more information
  • Selector Plant
    • Seeds and Tubers

      Our product microLYSIS®-Plus has successfully been used to extract PCR-ready DNA from a variety of seeds and tubers. For example, from peanuts, rice, grass seed – even the starchy endosperm of maize seeds. It can generate PCR-ready DNA in minutes which can be used immediately or stored long-term at -20°C

      For purified DNA from seeds/tubers we recommend our DNAMITE® Plant kits. We offer Kit#1 without RNAse and Kit#2 with, depending on your requirements

      Contact us for more information
    • Leaves, Stems and Flowers

      microLYSIS®-Plus has been used to generate PCR-ready DNA from the leaves, flower petals, pollen and tissues of many crop plants such as lettuce, pepper and paw-paw. It has also been used with research plants such as wheat (Triticum aestivum) and maize (Zea mays) and Arabidopsis thaliana. microLYSIS®-Plus generates PCR-ready DNA which can be used immediately or stored long-term at -20°C

      For purified DNA from plant leaves, flowers, pollen and tissue we recommend our DNAMITE® Plant kits. We offer kits with/without RNAse, depending on your needs

      Contact us for more information
  • Selector Fungi
    • Unicellular fungi

      Our customers report that microLYSIS® is effective for DNA release from the unicellular fungi Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida spp. This product releases PCR-ready DNA suitable for immediate use or long-term storage at -20°C. For purified DNA from these species we recommend our DNAMITE® Bacterial/Plasmid kit. If you are trialling DNA release or extraction from a different unicellular fungus, contact our technical support for advice, although in most cases we would expect the kits above to be sufficient

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    • Fungal spores

      microLYSIS®-Plus has been used to produce PCR-ready DNA from the spores of a variety of fungi. It has been used successfully with isolated spores of Phakospora euvitis (Grapevine Leaf Rust), leaf-borne spores of Sclerotinia sclerotiorum (Sclerotinia stem rot in oilseed rape) and with airborne spores, via spore-tape experiments, of the wheat pathogens Microdochium nivale cv. Nivale and Fusarium spp.

      microLYSIS®-Plus produces PCR-ready DNA which can be used immediately or stored long-term at -20°C

      Contact us for more information
    • Other fungi

      For production of PCR-ready DNA we recommend microLYSIS®-Plus. This product has been used with mycelial samples of a wide variety of fungal species. Notable examples include the novel bat pathogen Geomyces destructans and the nematophagus fungus Pochonia chlamydosporia. To produce purified DNA from fungal samples we recommend our DNAMITE® Plant Kit #2

      Contact us for more information
  • Selector Protist
    • Algae, slime moulds and water moulds

      Although we have customers that have used microLYSIS®-Plus to release PCR-ready DNA from the water mould oomycete Pythium aphanidermatum, as yet we have no customers using any of our products to extract DNA from either algae or slime moulds. However, we have tested our microLYSIS®-Plus solution on the slime mould Physarum polycephalum with some success. microLYSIS®-Plus disrupts cells to release DNA that is immediately suitable for PCR and real-time PCR. This PCR-ready DNA can be stored long-term at -20°C

      We anticipate that for most unicellular algae and other slime mould species, microLYSIS®-Plus would be sufficient for generating PCR-ready DNA. For more complex algal species, including seaweeds, a modified protocol may be successful or our DNAMITE® for Difficult Cells kit. We would also recommend this kit for those wishing to generate purified DNA from algae or slime moulds

      Contact us for more information
    • Cryptosporidium

      We have successfully trialled microLYSIS®-Plus for the extraction of PCR-ready DNA from the protozoan Cryptosporidium parvum. This product disintegrates the cell to release PCR-ready DNA which can either be used straight away or stored long term at-20°C. Customers wishing to generate purified DNA should use our DNAMITE® for Difficult Cells kit

      Contact us for more information
    • Other Protists

      The Protista kingdom is very diverse and our products have, of course, only been used with a tiny fraction of its species. For those organisms where there is not a tried and tested method we would recommend trialling microLYSIS®-Plus for PCR-ready DNA and our DNAMITE® for Difficult Cells kit for producing purified DNA. Alternatively, contact us for technical support and we’ll happily provide advice

      Contact us for more information
  • Selector Micro-organism
    • Gram-negative bacteria

      Our customers report 100% success lysing Gram-negative colony isolates with microLYSIS®. Notable species with which microLYSIS® has been used include Haemophilus influenzae and Neisseria meningitides during epidemiological screens.

      microLYSIS® disintegrates cells so that DNA is released (genomic and plasmid) which is immediately suitable for PCR and real-time PCR. This PCR-ready DNA can be stored long-term at -20°C.

      If customers require purified DNA from Gram-negative cells we suggest using our DNAMITE® Bacterial/Plasmid kit.

      Contact us for more information
    • Gram-positive bacteria

      Customers report almost 100% success lysing Gram-positive colony isolates with microLYSIS®-Plus. Notable species include Streptococcus pneumoniae and Bacillus cereus. microLYSIS®-Plus has also been used successfully in a general screen on colonies isolated from seawater, lysing enterococci as well as Bacteroides and coliforms.

      One significant advantage of microLYSIS®-Plus over some methods is that it leaves the genomic DNA largely intact. Thus, it has been shown to improve DNA collection from Gram-positive spore-forming micro-organisms where a bead-beating system would be the usual alternative method and when whole genome amplification is the objective.

      We always recommend microLYSIS®-Plus first to customers working with Gram-positive bacteria. It can disrupt tough cells to release DNA which is immediately suitable for PCR and real-time PCR. The DNA can also be stored long-term at -20°C. For the few Gram-positives which stubbornly resist this product (for example, DNA yields from S. agalactiae can be a little low) we recommend our DNAMITE® for Difficult Cells kit. We also recommend this kit if customers require purified DNA from gram-positive cells.

      Contact us for more information
    • Archaea

      As far as we know, we have no customers who specifically work with Archaeal species, so whilst it’s likely that Archaeal species that stain gram-negative could be lysed with microLYSIS®, we recommend microLYSIS®-Plus for obtaining PCR-ready DNA because of the great diversity of Archaeal cell wall structures.

      Like microLYSIS®, microLYSIS®-Plus disintegrates cells to release DNA, but unlike the former it is optimised to disrupt even the toughest cells. microLYSIS®-Plus disintegrates cells so that DNA is released (genomic and plasmid) which is immediately suitable for PCR and real-time PCR. This PCR-ready DNA can be stored long-term at -20°C.

      For customers that require purified DNA from Archaea we would recommend our DNAMITE® for Difficult Cells, although for Archaea that stain gram-negative we would expect our DNAMITE® Bacterial/Plasmid kit to be sufficient.

      Contact us for more information
    • Viruses

      Our novel column-free DNA release method microLYSIS®-Plus has been successfully optimised for Baculovirus. It delivers high yield complementing qPCR assays for quantitation of virus titrations to ensure optimal recombinant protein production. This method will reduce the amount of cell handling required and prevent against any delay in the protein production process.
          Protocol
              If you have any further queries please do not hesitate to Contact us
          • Alternatively, you can search our citation table for the particular sample or cell type that you are interested in:

            Sample / Cell TypeProductProtocolArticleURL
            Actinomyces naeslundiimicroLYSISaccording to manufacturer's protocolEffect of the Environment on Genotypic Diversity of Actinomyces naeslundii and Streptococcus oralis in the Oral Biofilm
            Actinomyces naeslundiimicroLYSISaccording to manufacturer's protocolPhenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations
            Brevibacterium linensmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Campylobacter jejunimicroLYSISaccording to manufacturer's protocolAnalysis of gluconeogenic and anaplerotic enzymes in Campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase
            Campylobacter jejunimicroLYSISaccording to manufacturer's protocolContribution of the stereospecific methionine sulphoxide reductases MsrA and MsrB to oxidative and nitrosative stress resistance in the food-borne pathogen Campylobacter jejuni
            Campylobacter jejunimicroLYSISaccording to manufacturer's protocolGrowth of Campylobacter jejuni Supported by Respiration of Fumarate, Nitrate, Nitrite, Trimethylamine-N-Oxide, or Dimethyl Sulfoxide Requires Oxygen
            Campylobacter jejunimicroLYSISaccording to manufacturer's protocolL-Serine Catabolism via an Oxygen-Labile L-Serine Dehydratase Is Essential for Colonization of the Avian Gut by Campylobacter jejuni
            Campylobacter jejunimicroLYSISaccording to manufacturer's protocolReduction of fumarate, mesaconate and crotonate by Mfr, a novel oxygen-regulated periplasmic reductase in Campylobacter jejuni
            Campylobacter jejunimicroLYSISaccording to manufacturer's protocolThe Campylobacter jejuni Thiol Peroxidases Tpx and Bcp Both Contribute to Aerotolerance and Peroxide-Mediated Stress Resistance but Have Distinct Substrate Specificities
            Candidatus phytoplasma malimicroLYSISaccording to manufacturer's protocolEndophytic bacterial community living in roots of healthy and ‘Candidatus Phytoplasma mali’-infected apple (Malus domestica, Borkh.) trees
            Clostridium botulinummicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secReport of two unlinked cases of infant botulism in the UK in October 2007
            Corynebacterium caseimicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Enterococcus faecalismicroLYSISaccording to manufacturer's protocolCharacterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei
            Enterococcus faecalismicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Enterococcus faeciummicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Escherichia colimicroLYSIS-Plusaccording to manufacturer's protocolIntegrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics
            Escherichia colimicroLYSIS-Plus65 °C for 15 min, 96 °C for 2 min, 65 °C for 4 min, 96 ° C for 1 min, 65 °C for 1 min and 96 °C for 30 secMonolithic Centrifugal Microfluidic Platform For Bacteria Capture and Concentration, Lysis, Nucleic-Acid Amplification, and Real-Time Detection
            Escherichia colimicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secSdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity
            Formalin-Fixed Paraffin-Embedded Tissue (FFPE)microLYSISaccording to manufacturer's protocolComparative evaluation of PCR assays for the robust molecular detection of Mycobacterium avium subsp. Paratuberculosis
            Fungal isolatesmicroLYSIS-Plusaccording to manufacturer's protocolFood for thought: Risks of non-native species transfer to the Antarctic region with fresh produce
            Fungal isolatesmicroLYSIS-Plusaccording to manufacturer's protocolGeomyces destructans sp. nov. associated with bat white-nose syndrome
            LactobacillimicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secFermented goats’ milk produced with selected multiple starters as a potentially functional food
            LactobacillimicroLYSISaccording to manufacturer's protocolIn situ activity of a bacteriocin-producing Lactococcus lactis strain. Influence on the interactions between lactic acid bacteria during sourdough fermentation
            LactobacillimicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secRapid Differentiation and In Situ Detection of 16 Sourdough Lactobacillus Species by Multiplex PCR
            LactobacillimicroLYSISaccording to manufacturer's protocolSurvival of Yogurt Bacteria in the Human Gut
            LactobacillimicroLYSISaccording to manufacturer's protocolAntibiotic resistance and microbial composition along the manufacturing process of Mozzarella di Bufala Campana
            Lactobacillus paracaseimicroLYSISaccording to manufacturer's protocolCharacterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei
            Lactobacillus acidipiscismicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Lactobacillus curvatusmicroLYSISaccording to manufacturer's protocolDirect Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef
            Lactobacillus curvatusmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Lactobacillus delbrueckiimicroLYSISaccording to manufacturer's protocolSurvival of Yogurt Bacteria in the Human Gut
            Lactobacillus plantarummicroLYSISaccording to manufacturer's protocolDisruption of the gene encoding glutamate dehydrogenase affects growth, amino acids catabolism and survival of Lactobacillus plantarum UC1001
            Lactobacillus sakeimicroLYSISaccording to manufacturer's protocolDirect Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef
            Leuconostoc gelidummicroLYSISaccording to manufacturer's protocolDirect Molecular Approach to Monitoring Bacterial Colonization on Vacuum-Packaged Beef
            Leuconostoc mesenteroidesmicroLYSISaccording to manufacturer's protocolCharacterization of the Tn916 Conjugative Transposon in a Food-Borne Strain of Lactobacillus paracasei
            Macrococcus caseolyticusmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Micrococcaceae strainsmicroLYSISaccording to manufacturer's protocolMonitoring the bacterial population dynamics during fermentation of artisanal Argentinean sausages
            Micrococcus luteusmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Microcystis sppmicroLYSISaccording to manufacturer's protocolThe diversity and distribution of toxigenic Microcystis spp. in present day and archived pelagic and sediment samples from Lake Erie
            Mouse tissuemicroLYSISaccording to manufacturer's protocolEarly-onset liver mtDNA depletion and late-onset proteinuric nephropathy in Mpv17 knockout mice
            Mycobacterium bovismicroLYSIS (20x)according to manufacturer's protocolAn extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens
            Mycobacterium smegmatismicroLYSISaccording to manufacturer's protocolA Kinetic Study of In Vitro Lysis of Mycobacterium smegmatis
            Neisseria meningitidismicroLYSISaccording to manufacturer's protocolConsecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease
            Neisseria meningitidismicroLYSISaccording to manufacturer's protocolMultilocus restriction typing: a tool for Neisseria meningitidis strain discrimination
            NematodemicroLYSIS-Plus (5x)66 °C for 30 min, 96 °C for 3 min, 65 °C for 4 min, 96 °C for 2 min, 65 °C for 1 min and 96 °C for 1 min; or incubation at 65 °C for 60 min and 94 °C for 10 minHsp90 gene, an additional target for discrimination between the potato cyst nematodes, Globodera rostochiensis and G. pallida, and the related species, G. tabacum tabacum
            PlanktonmicroLYSISaccording to manufacturer's protocolMicrocystin-Production in Lake Erie: Insight into Past and Present Populations
            Propionibacterium freudenrechiimicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Pseudomonas aeruginosamicroLYSISaccording to manufacturer's protocolComparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens- and non-contact lens-related keratitis
            Pseudomonas aeruginosamicroLYSISaccording to manufacturer's protocolEvaluation of an impedance method for subtyping of Pseudomonas aeruginosa
            Pseudomonas aeruginosamicroLYSISaccording to manufacturer's protocolPhenotypic Characterization of Clonal and Nonclonal Pseudomonas aeruginosa Strains Isolated from Lungs of Adults with Cystic Fibrosis
            Pseudomonas aeruginosamicroLYSISaccording to manufacturer's protocolProtease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis
            Pseudomonas aeruginosamicroLYSISaccording to manufacturer's protocolPseudomonas aeruginosa with LasI Quorum-Sensing Deficiency during Corneal Infection
            Pythium aphanidermatummicroLYSIS-Plus65 °C for 30 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secOccurrence of Pythium aphanidermatum Root and Collar Rot of Papaya (Carica papaya L.) in Cote d'Ivoire
            Soil microbesmicroLYSISaccording to manufacturer's protocolRhizodeposits of Trifolium pratense and Lolium perenne: their comparative effects on 2,4-D mineralization in two contrasting soils
            SpermentozamicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secLaser Microdissection Separation of Pure Spermatozoa Populations from Mixed Cell Samples for Forensic DNA Analysis
            Staphylococcus aureusmicroLYSISaccording to manufacturer's protocolDevelopment of a real-time quadruplex PCR assay for simultaneous detection of nuc, Panton–Valentine leucocidin (PVL), mecA and homologue mecALGA251
            Staphylococcus aureusmicroLYSISaccording to manufacturer's protocolPCR–DGGE analysis for the identification of microbial populations from Argentinean dry fermented sausages
            Staphylococcus capitismicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secSdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity
            Staphylococcus capraemicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Staphylococcus epidermidismicroLYSIS65 °C for 5 min, 96 °C for 2 min, 65 °C for 4 min, 96 °C for 1 min, 65 °C for 1 min and 96 °C for 30 secSdrX, a Serine-Aspartate Repeat Protein Expressed by Staphylococcus capitis with Collagen VI Binding Activity
            Staphylococcus equorummicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Staphylococcus lentusmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Staphylococcus saprophyticusmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Staphylococcus sciurimicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Staphylococcus vitulinusmicroLYSISaccording to manufacturer's protocolSurface microbiota analysis of Taleggio, Gorgonzola, Casera, Scimudin and Formaggio di Fossa Italian cheeses
            Streptococcus oralismicroLYSISaccording to manufacturer's protocolEffect of the Environment on Genotypic Diversity of Actinomyces naeslundii and Streptococcus oralis in the Oral Biofilm
            Streptococcus pneumoniaemicroLYSIS-Plusaccording to manufacturer's protocolMultiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes
            Streptococcus thermophilusmicroLYSISaccording to manufacturer's protocolSurvival of Yogurt Bacteria in the Human Gut
            Streptococcus thermophilusmicroLYSISaccording to manufacturer's protocolSafety aspects, genetic diversity and technological characterisation of wild-type Streptococcus thermophilus strains isolated from north Italian traditional cheeses
            VeillonellamicroLYSISaccording to manufacturer's protocolRapid Succession within the Veillonella Population of a Developing Human Oral Biofilm In Situ